image acquisition image processing toolboxes release 2015a Search Results


influenza a h1n1 (a/michigan/45/2015) neuraminidase / na  (Sino Biological)
93
Sino Biological influenza a h1n1 (a/michigan/45/2015) neuraminidase / na
Influenza A H1n1 (A/Michigan/45/2015) Neuraminidase / Na, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ni-catalyzed reactions  (Solvias AG)
90
Solvias AG ni-catalyzed reactions
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incucyte zoom microscope software 2015a  (Sartorius AG)
99
Sartorius AG incucyte zoom microscope software 2015a
Late passage astrocytes show reduced mitochondrial membrane potential and ATP production. (A) Astrocytes were conducted for mitochondrial membrane potential by fluorescence staining of TMRM. Representative images were obtained by the IncuCyte ZOOM microscope software <t>2015A</t> (Essen Bioscience). The data were measured by IncuCyte software (Essen Bioscience). The representative graph shows the total TMRM object integrated intensity (RCU x μm 2 /image) and the total TMRM object area (μm 2 /image). Bars represent the mean ± SEM (n=3). **indicates p <0.01, and ***indicates p <0.001 vs. vehicle group. (B) The mitochondrial OCR of astrocytes was experimented using Cell Mito Test Kit and Agilent Seahorse XFe96 Analyzer (Agilent Technologies). The representative graphs were visualized using Wave Desktop 2.6 software (Agilent Technologies). The graphs represent the basal level, spare respiratory capacity, proton leak, and ATP production level. OCRs are mean ± SEM (n=3), normalized to the third measurement point and expressed as % baseline OCR. *indicates p <0.05 and, ***indicates p <0.001 vs. P1 astrocytes.
Incucyte Zoom Microscope Software 2015a, supplied by Sartorius AG, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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the annihilation of nature: human extinction of birds and mammals  (Johns Hopkins HealthCare)
90
Johns Hopkins HealthCare the annihilation of nature: human extinction of birds and mammals
Late passage astrocytes show reduced mitochondrial membrane potential and ATP production. (A) Astrocytes were conducted for mitochondrial membrane potential by fluorescence staining of TMRM. Representative images were obtained by the IncuCyte ZOOM microscope software <t>2015A</t> (Essen Bioscience). The data were measured by IncuCyte software (Essen Bioscience). The representative graph shows the total TMRM object integrated intensity (RCU x μm 2 /image) and the total TMRM object area (μm 2 /image). Bars represent the mean ± SEM (n=3). **indicates p <0.01, and ***indicates p <0.001 vs. vehicle group. (B) The mitochondrial OCR of astrocytes was experimented using Cell Mito Test Kit and Agilent Seahorse XFe96 Analyzer (Agilent Technologies). The representative graphs were visualized using Wave Desktop 2.6 software (Agilent Technologies). The graphs represent the basal level, spare respiratory capacity, proton leak, and ATP production level. OCRs are mean ± SEM (n=3), normalized to the third measurement point and expressed as % baseline OCR. *indicates p <0.05 and, ***indicates p <0.001 vs. P1 astrocytes.
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angewandte chemie  (Chemie GmbH)
90
Chemie GmbH angewandte chemie
Late passage astrocytes show reduced mitochondrial membrane potential and ATP production. (A) Astrocytes were conducted for mitochondrial membrane potential by fluorescence staining of TMRM. Representative images were obtained by the IncuCyte ZOOM microscope software <t>2015A</t> (Essen Bioscience). The data were measured by IncuCyte software (Essen Bioscience). The representative graph shows the total TMRM object integrated intensity (RCU x μm 2 /image) and the total TMRM object area (μm 2 /image). Bars represent the mean ± SEM (n=3). **indicates p <0.01, and ***indicates p <0.001 vs. vehicle group. (B) The mitochondrial OCR of astrocytes was experimented using Cell Mito Test Kit and Agilent Seahorse XFe96 Analyzer (Agilent Technologies). The representative graphs were visualized using Wave Desktop 2.6 software (Agilent Technologies). The graphs represent the basal level, spare respiratory capacity, proton leak, and ATP production level. OCRs are mean ± SEM (n=3), normalized to the third measurement point and expressed as % baseline OCR. *indicates p <0.05 and, ***indicates p <0.001 vs. P1 astrocytes.
Angewandte Chemie, supplied by Chemie GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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2015a  (MathWorks Inc)
96
MathWorks Inc 2015a
Late passage astrocytes show reduced mitochondrial membrane potential and ATP production. (A) Astrocytes were conducted for mitochondrial membrane potential by fluorescence staining of TMRM. Representative images were obtained by the IncuCyte ZOOM microscope software <t>2015A</t> (Essen Bioscience). The data were measured by IncuCyte software (Essen Bioscience). The representative graph shows the total TMRM object integrated intensity (RCU x μm 2 /image) and the total TMRM object area (μm 2 /image). Bars represent the mean ± SEM (n=3). **indicates p <0.01, and ***indicates p <0.001 vs. vehicle group. (B) The mitochondrial OCR of astrocytes was experimented using Cell Mito Test Kit and Agilent Seahorse XFe96 Analyzer (Agilent Technologies). The representative graphs were visualized using Wave Desktop 2.6 software (Agilent Technologies). The graphs represent the basal level, spare respiratory capacity, proton leak, and ATP production level. OCRs are mean ± SEM (n=3), normalized to the third measurement point and expressed as % baseline OCR. *indicates p <0.05 and, ***indicates p <0.001 vs. P1 astrocytes.
2015a, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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crispr-cas9 genome editing  (Broad Institute Inc)
90
Broad Institute Inc crispr-cas9 genome editing
Late passage astrocytes show reduced mitochondrial membrane potential and ATP production. (A) Astrocytes were conducted for mitochondrial membrane potential by fluorescence staining of TMRM. Representative images were obtained by the IncuCyte ZOOM microscope software <t>2015A</t> (Essen Bioscience). The data were measured by IncuCyte software (Essen Bioscience). The representative graph shows the total TMRM object integrated intensity (RCU x μm 2 /image) and the total TMRM object area (μm 2 /image). Bars represent the mean ± SEM (n=3). **indicates p <0.01, and ***indicates p <0.001 vs. vehicle group. (B) The mitochondrial OCR of astrocytes was experimented using Cell Mito Test Kit and Agilent Seahorse XFe96 Analyzer (Agilent Technologies). The representative graphs were visualized using Wave Desktop 2.6 software (Agilent Technologies). The graphs represent the basal level, spare respiratory capacity, proton leak, and ATP production level. OCRs are mean ± SEM (n=3), normalized to the third measurement point and expressed as % baseline OCR. *indicates p <0.05 and, ***indicates p <0.001 vs. P1 astrocytes.
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doppler boundary layer lidars  (Scalex Corporation)
90
Scalex Corporation doppler boundary layer lidars
Late passage astrocytes show reduced mitochondrial membrane potential and ATP production. (A) Astrocytes were conducted for mitochondrial membrane potential by fluorescence staining of TMRM. Representative images were obtained by the IncuCyte ZOOM microscope software <t>2015A</t> (Essen Bioscience). The data were measured by IncuCyte software (Essen Bioscience). The representative graph shows the total TMRM object integrated intensity (RCU x μm 2 /image) and the total TMRM object area (μm 2 /image). Bars represent the mean ± SEM (n=3). **indicates p <0.01, and ***indicates p <0.001 vs. vehicle group. (B) The mitochondrial OCR of astrocytes was experimented using Cell Mito Test Kit and Agilent Seahorse XFe96 Analyzer (Agilent Technologies). The representative graphs were visualized using Wave Desktop 2.6 software (Agilent Technologies). The graphs represent the basal level, spare respiratory capacity, proton leak, and ATP production level. OCRs are mean ± SEM (n=3), normalized to the third measurement point and expressed as % baseline OCR. *indicates p <0.05 and, ***indicates p <0.001 vs. P1 astrocytes.
Doppler Boundary Layer Lidars, supplied by Scalex Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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automation products  (Panasonic Healthcare)
90
Panasonic Healthcare automation products
Late passage astrocytes show reduced mitochondrial membrane potential and ATP production. (A) Astrocytes were conducted for mitochondrial membrane potential by fluorescence staining of TMRM. Representative images were obtained by the IncuCyte ZOOM microscope software <t>2015A</t> (Essen Bioscience). The data were measured by IncuCyte software (Essen Bioscience). The representative graph shows the total TMRM object integrated intensity (RCU x μm 2 /image) and the total TMRM object area (μm 2 /image). Bars represent the mean ± SEM (n=3). **indicates p <0.01, and ***indicates p <0.001 vs. vehicle group. (B) The mitochondrial OCR of astrocytes was experimented using Cell Mito Test Kit and Agilent Seahorse XFe96 Analyzer (Agilent Technologies). The representative graphs were visualized using Wave Desktop 2.6 software (Agilent Technologies). The graphs represent the basal level, spare respiratory capacity, proton leak, and ATP production level. OCRs are mean ± SEM (n=3), normalized to the third measurement point and expressed as % baseline OCR. *indicates p <0.05 and, ***indicates p <0.001 vs. P1 astrocytes.
Automation Products, supplied by Panasonic Healthcare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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winfolia pro 2015a software  (Regent Instruments)
86
Regent Instruments winfolia pro 2015a software
Late passage astrocytes show reduced mitochondrial membrane potential and ATP production. (A) Astrocytes were conducted for mitochondrial membrane potential by fluorescence staining of TMRM. Representative images were obtained by the IncuCyte ZOOM microscope software <t>2015A</t> (Essen Bioscience). The data were measured by IncuCyte software (Essen Bioscience). The representative graph shows the total TMRM object integrated intensity (RCU x μm 2 /image) and the total TMRM object area (μm 2 /image). Bars represent the mean ± SEM (n=3). **indicates p <0.01, and ***indicates p <0.001 vs. vehicle group. (B) The mitochondrial OCR of astrocytes was experimented using Cell Mito Test Kit and Agilent Seahorse XFe96 Analyzer (Agilent Technologies). The representative graphs were visualized using Wave Desktop 2.6 software (Agilent Technologies). The graphs represent the basal level, spare respiratory capacity, proton leak, and ATP production level. OCRs are mean ± SEM (n=3), normalized to the third measurement point and expressed as % baseline OCR. *indicates p <0.05 and, ***indicates p <0.001 vs. P1 astrocytes.
Winfolia Pro 2015a Software, supplied by Regent Instruments, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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influenza a h1n1 (a/michigan/45/2015) nucleoprotein / np protein  (Sino Biological)
95
Sino Biological influenza a h1n1 (a/michigan/45/2015) nucleoprotein / np protein
Late passage astrocytes show reduced mitochondrial membrane potential and ATP production. (A) Astrocytes were conducted for mitochondrial membrane potential by fluorescence staining of TMRM. Representative images were obtained by the IncuCyte ZOOM microscope software <t>2015A</t> (Essen Bioscience). The data were measured by IncuCyte software (Essen Bioscience). The representative graph shows the total TMRM object integrated intensity (RCU x μm 2 /image) and the total TMRM object area (μm 2 /image). Bars represent the mean ± SEM (n=3). **indicates p <0.01, and ***indicates p <0.001 vs. vehicle group. (B) The mitochondrial OCR of astrocytes was experimented using Cell Mito Test Kit and Agilent Seahorse XFe96 Analyzer (Agilent Technologies). The representative graphs were visualized using Wave Desktop 2.6 software (Agilent Technologies). The graphs represent the basal level, spare respiratory capacity, proton leak, and ATP production level. OCRs are mean ± SEM (n=3), normalized to the third measurement point and expressed as % baseline OCR. *indicates p <0.05 and, ***indicates p <0.001 vs. P1 astrocytes.
Influenza A H1n1 (A/Michigan/45/2015) Nucleoprotein / Np Protein, supplied by Sino Biological, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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matlab 2015  (MathWorks Inc)
96
MathWorks Inc matlab 2015
Late passage astrocytes show reduced mitochondrial membrane potential and ATP production. (A) Astrocytes were conducted for mitochondrial membrane potential by fluorescence staining of TMRM. Representative images were obtained by the IncuCyte ZOOM microscope software <t>2015A</t> (Essen Bioscience). The data were measured by IncuCyte software (Essen Bioscience). The representative graph shows the total TMRM object integrated intensity (RCU x μm 2 /image) and the total TMRM object area (μm 2 /image). Bars represent the mean ± SEM (n=3). **indicates p <0.01, and ***indicates p <0.001 vs. vehicle group. (B) The mitochondrial OCR of astrocytes was experimented using Cell Mito Test Kit and Agilent Seahorse XFe96 Analyzer (Agilent Technologies). The representative graphs were visualized using Wave Desktop 2.6 software (Agilent Technologies). The graphs represent the basal level, spare respiratory capacity, proton leak, and ATP production level. OCRs are mean ± SEM (n=3), normalized to the third measurement point and expressed as % baseline OCR. *indicates p <0.05 and, ***indicates p <0.001 vs. P1 astrocytes.
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Image Search Results


Late passage astrocytes show reduced mitochondrial membrane potential and ATP production. (A) Astrocytes were conducted for mitochondrial membrane potential by fluorescence staining of TMRM. Representative images were obtained by the IncuCyte ZOOM microscope software 2015A (Essen Bioscience). The data were measured by IncuCyte software (Essen Bioscience). The representative graph shows the total TMRM object integrated intensity (RCU x μm 2 /image) and the total TMRM object area (μm 2 /image). Bars represent the mean ± SEM (n=3). **indicates p <0.01, and ***indicates p <0.001 vs. vehicle group. (B) The mitochondrial OCR of astrocytes was experimented using Cell Mito Test Kit and Agilent Seahorse XFe96 Analyzer (Agilent Technologies). The representative graphs were visualized using Wave Desktop 2.6 software (Agilent Technologies). The graphs represent the basal level, spare respiratory capacity, proton leak, and ATP production level. OCRs are mean ± SEM (n=3), normalized to the third measurement point and expressed as % baseline OCR. *indicates p <0.05 and, ***indicates p <0.001 vs. P1 astrocytes.

Journal: Biomolecules & Therapeutics

Article Title: Late Passage Cultivation Induces Aged Astrocyte Phenotypes in Rat Primary Cultured Cells

doi: 10.4062/biomolther.2020.175

Figure Lengend Snippet: Late passage astrocytes show reduced mitochondrial membrane potential and ATP production. (A) Astrocytes were conducted for mitochondrial membrane potential by fluorescence staining of TMRM. Representative images were obtained by the IncuCyte ZOOM microscope software 2015A (Essen Bioscience). The data were measured by IncuCyte software (Essen Bioscience). The representative graph shows the total TMRM object integrated intensity (RCU x μm 2 /image) and the total TMRM object area (μm 2 /image). Bars represent the mean ± SEM (n=3). **indicates p <0.01, and ***indicates p <0.001 vs. vehicle group. (B) The mitochondrial OCR of astrocytes was experimented using Cell Mito Test Kit and Agilent Seahorse XFe96 Analyzer (Agilent Technologies). The representative graphs were visualized using Wave Desktop 2.6 software (Agilent Technologies). The graphs represent the basal level, spare respiratory capacity, proton leak, and ATP production level. OCRs are mean ± SEM (n=3), normalized to the third measurement point and expressed as % baseline OCR. *indicates p <0.05 and, ***indicates p <0.001 vs. P1 astrocytes.

Article Snippet: We detected the red fluorescence using the IncuCyte ZOOM system (Essen Bioscience) by imaging each well every 30 min for 12 h and analyzed the red particles using the IncuCyte ZOOM microscope software 2015A (Essen Bioscience).

Techniques: Membrane, Fluorescence, Staining, Microscopy, Software